Description
Specifications Table
| Product Name | Tris(hydroxymethyl)aminomethane, 99.8%, ACS reagent |
|---|---|
| Pack Size | 100GR, 500GR, 2.5KG |
| Form | Powder |
| Grade | ACS Reagent |
| Application | Buffer preparation, molecular biology, protein research |
Product Description
Tris(hydroxymethyl)aminomethane, commonly known as Tris base, is a high-purity buffer reagent essential for maintaining stable pH in biological and biochemical applications. With a purity of 99.8% and ACS-grade certification, this product is ideal for researchers, students, and educators working in molecular biology, protein studies, and DNA/RNA analysis.
Tris buffer is widely used in electrophoresis, cell culture media, and enzyme assays due to its excellent buffering capacity in the physiological pH range (7.0–9.0). Its low reactivity with most biological molecules makes it a preferred choice for sensitive experiments.
At eqipped, we ensure that this Tris base meets stringent quality standards, providing consistent performance in your lab experiments. Whether you’re preparing Tris-HCl buffers or conducting protein purification, this reagent delivers reliable results every time.
Need high-quality Tris buffer for your research? Buy Tris(hydroxymethyl)aminomethane online at eqipped today.
Key Applications
Tris base is a versatile reagent used in various laboratory applications, including:
- DNA and RNA electrophoresis buffers
- Protein purification and crystallization
- Cell culture media preparation
- Enzyme assays and biochemical reactions
- pH adjustment in biological solutions
Why Tris Buffer is Essential in Labs
Tris buffer is a staple in molecular biology labs because of its ability to maintain a stable pH environment. Unlike other buffers, Tris has a pKa close to physiological pH, making it suitable for experiments involving proteins and nucleic acids. Its water solubility and low toxicity further enhance its utility in sensitive applications.
When purchasing Tris base, always opt for high-purity grades like this ACS-certified product to avoid contaminants that could interfere with your results. Eqipped offers this Tris reagent in convenient pack sizes (100GR, 500GR, 2.5KG) to suit both small-scale and bulk requirements.
Safety and Handling
While Tris base is generally safe to handle, it is recommended to wear appropriate personal protective equipment (PPE) such as gloves and goggles when working with the powder. Store the product in a cool, dry place away from moisture to prevent degradation.
FAQs
What is the difference between Tris base and Tris-HCl?
Tris base is the free amine form, while Tris-HCl is the hydrochloride salt. Tris base is used to prepare buffers by adjusting the pH with HCl.
Can I use this Tris base for DNA electrophoresis?
Yes, this 99.8% pure Tris base is ideal for preparing TAE and TBE buffers used in DNA electrophoresis.
What is the shelf life of Tris(hydroxymethyl)aminomethane?
When stored properly in a sealed container, Tris base has a shelf life of 2–3 years.
Is this product suitable for cell culture applications?
Absolutely. The ACS-grade purity ensures minimal contaminants, making it safe for cell culture media preparation.
How do I prepare a 1M Tris buffer solution?
Dissolve 121.14 grams of Tris base in 800 mL of water, adjust the pH with HCl, and bring the volume to 1 liter.
