Specifications Table

Product Name – Tris(hydroxymethyl)aminomethane, 99.8%, for analysis, biochemical grade
Quantity/Pack Size – 100GR, 1KG, 5KG
Form – Powder
Grade – Biochemical, 99.8% purity
Application – Buffering agent in molecular biology, protein research

Product Overview

Tris(hydroxymethyl)aminomethane, commonly known as Tris buffer, is a highly refined biochemical reagent with 99.8% purity, specifically designed for analytical and research applications. This white, crystalline powder serves as a critical buffering agent in molecular biology, biochemistry, and protein research due to its excellent pH stability in the physiological range (7.0–9.0). Its high solubility in water and low reactivity with most biological systems make it indispensable for preparing stable solutions in electrophoresis, cell culture, and enzyme assays. The biochemical grade ensures minimal impurities, reducing interference in sensitive experiments. Unlike other buffers, Tris maintains consistent performance across a wide temperature range, making it ideal for protocols requiring precise pH control. Its non-toxic and non-volatile nature further enhances safety in lab environments. Whether used in DNA/RNA extraction, protein purification, or as a component in cell lysis buffers, this Tris variant guarantees reproducibility and reliability in experimental outcomes.

FAQs

1. What is the exact pH range where Tris buffer is most effective?

Tris buffer functions optimally between pH 7.0 and 9.0, with its highest buffering capacity around pH 8.06 at 25°C. The pKa value is 8.06, making it suitable for most biological applications within this range.

2. Can this Tris buffer be used for cell culture media preparation?

Yes, this 99.8% pure Tris buffer is compatible with cell culture media, provided it is sterilized via filtration or autoclaving before use. Its biochemical grade ensures minimal cytotoxicity, supporting cell viability.

3. How does the purity of 99.8% impact experimental results compared to lower grades?

The 99.8% purity reduces the risk of contamination from metals, nucleases, or proteases, which can degrade biomolecules or skew results. Lower grades may introduce artifacts in sensitive assays like PCR or sequencing.

4. What is the recommended storage condition to maintain stability?

Store the powder in a tightly sealed container at room temperature (15–25°C), protected from moisture and light. Prepared Tris solutions should be refrigerated (2–8°C) and used within 1–2 weeks to prevent microbial growth.

5. Are there any known incompatibilities with other common lab reagents?

Tris buffer may react with aldehydes (e.g., formaldehyde) and certain metal ions (e.g., Cu²⁺, Fe³⁺), forming insoluble complexes. Avoid mixing with strong acids or bases without prior pH adjustment.