Description
Specifications Table
Product Name – Tris(hydroxymethyl)aminomethane, 99.85%, for molecular biology, DNAse, RNAse and Protease free
Quantity/Pack Size – 1KG, 5KG
Form – Powder
Grade – Molecular Biology Grade
Application – Buffer preparation, nucleic acid research, protein studies
Product Overview
Tris(hydroxymethyl)aminomethane, commonly known as Tris Base, is a highly purified biochemical reagent with a minimum assay of 99.85%, specifically designed for molecular biology applications. This grade is rigorously tested to ensure it is free from DNAse, RNAse, and protease contaminants, making it ideal for sensitive laboratory procedures where enzymatic activity could compromise results. The compound serves as a critical buffering agent in biological systems, maintaining stable pH levels across a wide range of experimental conditions. Its high solubility in water and compatibility with various biological molecules make it a staple in protocols involving nucleic acid extraction, electrophoresis, and protein purification. The molecular biology grade ensures consistency and reliability in research applications where precision is paramount. This product undergoes stringent quality control measures to guarantee lot-to-lot uniformity, providing researchers with confidence in their experimental outcomes.
FAQs
1. What is the pH range of Tris Base in biological applications?
Tris Base has an effective buffering range between pH 7.0 and 9.2, making it particularly useful for most biological systems that operate within this physiological pH spectrum.
2. How should Tris Base be stored to maintain its purity?
Store Tris Base in a cool, dry place at room temperature, tightly sealed in its original container to prevent moisture absorption and contamination.
3. Is this Tris Base compatible with common laboratory detergents?
Yes, this molecular biology grade Tris Base is compatible with most non-ionic and mild ionic detergents commonly used in laboratory protocols.
4. What alternatives exist for applications requiring different pH ranges?
For applications outside Tris Base’s effective range, alternatives like HEPES (pH 6.8-8.2) or MES (pH 5.5-6.7) may be more appropriate depending on specific experimental requirements.
5. Does this product contain any animal-derived components?
No, this Tris Base is synthetically produced and does not contain any animal-derived components, making it suitable for vegan laboratory practices.
