Specifications Table

Product Name – Tris(hydroxymethyl)aminomethane, 99+%, for biochemistry
Quantity/Pack Size – 500GR, 1KG, 2.5KG, 10KG
Form – Crystalline powder
Grade – Biochemistry, 99%+ purity
Application – Buffer preparation, nucleic acid purification, protein electrophoresis

Product Overview

Tris(hydroxymethyl)aminomethane (Tris Base) is a high-purity (99%+) biochemical reagent designed for critical laboratory applications requiring precise pH control. This crystalline powder serves as a primary buffering agent in molecular biology protocols, offering superior stability across physiological pH ranges (7.0–9.0). Its zwitterionic nature at neutral pH minimizes ionic interference, making it indispensable for DNA/RNA extraction, gel electrophoresis, and enzyme assays. The reagent undergoes rigorous quality testing to ensure minimal heavy metal contamination (<5 ppm) and consistent lot-to-lot performance. Its high solubility in water (600 g/L at 25°C) facilitates rapid buffer preparation without residual particulates. The product’s low hygroscopicity preserves shelf stability under standard lab conditions (15–30°C), while its compatibility with common sterilization methods (autoclaving, filtration) enhances versatility. Each batch includes a certificate of analysis verifying purity, pH response, and endotoxin levels (<0.1 EU/mg), meeting ISO 9001:2015 standards for research-grade chemicals.

FAQs

1. What is the exact pKa of this Tris Base at 25°C?

The pKa of this Tris Base is 8.06 at 25°C, which is critical for calculating buffer concentrations in biological systems.

2. Can this product be used for cell culture media preparation?

While primarily designed for molecular biology, this grade meets purity requirements for cell culture buffers when supplemented with appropriate nutrients.

3. How does this compare to HEPES buffer in terms of temperature sensitivity?

Tris buffers exhibit greater temperature-dependent pH shifts (ΔpH/°C = -0.031) compared to HEPES (ΔpH/°C = -0.014), requiring temperature compensation in assays.

4. What’s the recommended storage condition to prevent degradation?

Store in a tightly sealed container at 15–30°C, protected from moisture and direct light to maintain stability for up to 36 months.

5. Is this product tested for DNase/RNase activity?

Yes, each batch undergoes enzymatic activity testing to confirm absence of DNase/RNase contamination, ensuring integrity in nucleic acid workflows.