Description
Specifications Table
| Product Name | Tris(hydroxymethyl)aminomethane hydrochloride |
|---|---|
| Pack Size | 100GR, 250GR, 1KG, 5KG |
| Form | Powder |
| Grade | Extra Pure (99%+) |
| Application | Molecular biology, buffer solutions, protein/DNA research |
Product Description
Tris hydrochloride (Tris HCl) is a cornerstone reagent in biochemistry and molecular biology labs. This extra pure grade (99%+) ensures reliable performance in critical applications like DNA electrophoresis, protein purification, and enzyme assays. Unlike Tris base, Tris HCl provides a pre-adjusted acidic form, simplifying buffer preparation for experiments requiring precise pH control between 7.0–9.0.
Researchers and students trust this high-purity powder for its consistency in creating Tris buffer solutions, essential for maintaining protein stability and nucleic acid integrity. The powder dissolves easily in water, forming clear solutions without residual impurities that could interfere with sensitive assays.
For labs prioritizing reproducibility, this Thermofisher-certified product meets rigorous quality standards. Whether you’re setting up a PCR reaction, running a gel, or optimizing a protein crystallization protocol, this Tris HCl delivers the purity needed for accurate results.
Need bulk quantities for your lab? eqipped offers competitive pricing on 1KG and 5KG packs—ideal for high-throughput research facilities.
Key Applications
This versatile buffer component is used in:
- DNA/RNA extraction and purification protocols
- SDS-PAGE and Western blotting buffers
- Cell culture media supplementation
- Enzyme activity assays requiring stable pH
- Protein crystallization screens
Tris Base vs. Tris HCl: What’s the Difference?
While both are derived from tris(hydroxymethyl)aminomethane, their forms differ:
Tris base is alkaline and requires hydrochloric acid for pH adjustment. Tris HCl, however, comes pre-acidified, offering convenience for labs needing immediate buffer preparation. For applications demanding tight pH control (e.g., 8.0 for DNA work), Tris HCl eliminates guesswork.
Safety and Handling
Risk Note: Tris HCl is generally safe but may cause mild skin/eye irritation. Always wear gloves and goggles when handling. Store in a cool, dry place away from moisture to prevent clumping.
FAQs
What is the shelf life of Tris HCl powder?
When stored properly in a sealed container, Tris HCl powder remains stable for 3–5 years. Avoid exposure to humidity to prevent degradation.
Can I use Tris HCl for PCR buffers?
Yes, Tris HCl is commonly used in PCR buffers to maintain optimal pH for Taq polymerase activity, typically at concentrations of 10–50 mM.
How do I prepare a 1M Tris HCl solution?
Dissolve 157.6 g of Tris HCl in 1L of distilled water, then adjust pH with HCl or NaOH as needed. Sterilize by autoclaving for molecular biology use.
Is this product suitable for cell culture?
Absolutely. The extra pure grade ensures minimal endotoxin contamination, making it safe for mammalian cell culture applications.
Where can I buy Tris HCl powder online in India?
eqipped offers authentic Thermofisher Tris HCl with fast shipping across India. Check our bulk discounts for research labs and universities.
