Description
Specifications Table
| Product Name | Trypsin 1:250 ex. Bovine Pancreas |
|---|---|
| Pack Size | 25 Gms, 100 Gms, 500 Gms |
| Form | Powder |
| Grade | 1:250 |
| Application | Cell culture, cell dissociation |
Product Description
Trypsin 1:250 derived from bovine pancreas is a highly purified enzyme essential for cell culture applications. With an activity of 1000BAEE U/mg and low endotoxin levels (10.0EU/mg), it ensures efficient cell dissociation while maintaining cell viability. This trypsin is widely used in research labs, biotech industries, and educational institutions for subculturing adherent cells, tissue dissociation, and primary cell isolation.
When working with trypsin for cell culture, it’s crucial to use a product that balances enzymatic activity with minimal contamination. This bovine pancreas trypsin meets stringent quality standards, making it a reliable choice for sensitive cellular applications. Whether you’re a student setting up your first cell culture experiment or a researcher optimizing protocols, this trypsin provides consistent performance.
For best results, reconstitute the powder in a balanced salt solution or culture medium, adjusting the concentration based on your specific cell line. Always follow standard aseptic techniques to prevent contamination. eqipped offers this trypsin in multiple pack sizes to suit different lab requirements, ensuring you have the right quantity for your experiments.
Upgrade your cell culture workflow with high-quality trypsin. Buy online today for fast delivery across India.
FAQs
What is the ideal concentration of trypsin for cell dissociation?
The optimal concentration varies by cell type, but 0.05% to 0.25% (w/v) in PBS or culture medium is commonly used. Always refer to your specific cell line’s protocol.
Can this trypsin be used for primary cell isolation?
Yes, this trypsin is suitable for primary cell isolation due to its high purity and controlled endotoxin levels, which help maintain cell viability.
How do I inactivate trypsin after dissociation?
Add an equal volume of culture medium containing serum (e.g., FBS) to neutralize trypsin activity. Alternatively, use a trypsin inhibitor solution.
Is this trypsin suitable for GMP-compliant processes?
While this product is high-quality, it is not specifically manufactured under GMP conditions. For GMP applications, consider certified alternatives.
What safety precautions should I take when handling trypsin?
Wear appropriate PPE, including gloves and lab coats. Avoid inhalation or direct contact with skin and eyes. Work in a well-ventilated area or biosafety cabinet.
