Description
Specifications Table
| Product Name | Trypsin (3x cryst.) ex Bovine Pancreas |
|---|---|
| Pack Size | 250 Mg, 1 Gms, 5 Gms |
| Form | Powder |
| Grade | 3x Crystallized |
| Application | Cell culture, tissue dissociation, research |
Product Description
Trypsin (3x crystallized) derived from bovine pancreas is a highly purified enzyme widely used in cell culture and tissue dissociation protocols. With an activity of 7500 BAEE U/mg (2500 NF U/mg), this trypsin powder ensures efficient cell detachment without compromising viability, making it a staple in laboratories across India.
Whether you’re a student setting up your first cell culture experiment or a researcher optimizing protocols, this trypsin enzyme offers consistency and reliability. Its high specificity for peptide bonds after lysine or arginine residues makes it indispensable for applications like primary cell isolation, sub-culturing, and recombinant protein production.
At eqipped, we understand the importance of quality reagents in research. That’s why we source this trypsin powder from trusted suppliers like SRL Chem, ensuring you receive a product that meets rigorous standards. The powder form allows for flexible reconstitution, letting you prepare working solutions tailored to your experimental needs.
Upgrade your cell culture workflow with premium trypsin powder. Buy online now at eqipped for seamless delivery across India.
FAQs
What is the difference between 3x crystallized trypsin and regular trypsin?
3x crystallized trypsin undergoes additional purification steps, resulting in higher purity and specific activity. This reduces batch-to-batch variability and minimizes non-specific proteolysis compared to less purified forms.
How do I reconstitute trypsin powder for cell culture?
Dissolve the powder in sterile, deionized water or a balanced salt solution like PBS or HBSS. A common working concentration is 0.25% (w/v), but optimize based on your cell type and protocol requirements.
Can this trypsin be used for primary cell isolation?
Yes, this high-purity trypsin is suitable for primary cell isolation from tissues. However, you may need to supplement it with collagenase or other enzymes depending on the tissue type.
What safety precautions should I take when handling trypsin?
Trypsin is a protease that can degrade proteins, including those in your skin and eyes. Always wear gloves, safety goggles, and a lab coat. In case of contact, rinse immediately with plenty of water.
How long can I store reconstituted trypsin solution?
Reconstituted trypsin can be stored at -20°C for up to 6 months or at 2-8°C for up to 1 month. For best results, aliquot the solution to avoid repeated freeze-thaw cycles.
