Description
Specifications Table
| Product Name | Trypsin Recombinant, Porcine Source: E. coli Cell Culture Tested | TC1485 | CDH |
|---|---|
| Pack Size | 25 mg, 100 mg, 1 gm |
| Form | Lyophilized powder |
| Grade | Cell Culture Tested |
| Application | Cell dissociation, sub-culturing, tissue dissociation |
Product Description
When it comes to cell culture research, the quality of your reagents can make or break your experiments. Trypsin recombinant, derived from porcine source and expressed in E. coli, is a highly purified enzyme designed specifically for cell dissociation applications. Unlike traditional trypsin sourced from animal pancreases, this recombinant version offers superior consistency, reduced risk of contamination, and batch-to-batch reliability—critical factors for researchers and educators working with sensitive cell lines.
This cell culture grade trypsin is ideal for detaching adherent cells from culture vessels without compromising viability. Whether you’re working with primary cells, stem cells, or established cell lines, this enzyme provides gentle yet effective dissociation, making it a staple in labs across India. The lyophilized form ensures long-term stability, while the sterile packaging maintains integrity until use.
At eqipped, we understand the demands of modern research. That’s why we source only the highest-grade reagents, ensuring you get products that meet international standards. Whether you’re a student setting up your first cell culture experiment or a seasoned researcher scaling up your work, this trypsin recombinant delivers the performance you need.
Upgrade your cell culture workflow with premium recombinant trypsin. Buy online now for seamless delivery to your lab.
FAQs
What is the difference between recombinant trypsin and animal-derived trypsin?
Recombinant trypsin is produced in E. coli using porcine trypsin genes, offering higher purity and consistency than animal-derived versions. It eliminates risks of batch variability and potential contaminants like viruses or other proteases.
Can this trypsin be used for primary cell isolation?
Yes, this cell culture grade trypsin is suitable for primary cell isolation, though the incubation time may need optimization based on tissue type. For delicate primary cells, consider using a lower concentration (0.025-0.05%).
What is the recommended working concentration for sub-culturing?
A 0.05% solution in PBS or HBSS is standard for most adherent cell lines. For sensitive cells, reduce to 0.025%. Always neutralize with serum-containing media after dissociation.
How should I reconstitute the lyophilized trypsin?
Dissolve the powder in sterile 1x PBS or HBSS without calcium/magnesium to the desired concentration. Filter sterilize if needed, then aliquot and store at -20°C.
Where can I buy recombinant trypsin online in India?
You can purchase this high-quality recombinant trypsin directly from eqipped. We offer multiple pack sizes (25 mg, 100 mg, 1 gm) with fast shipping across India. Order now to ensure your lab is always stocked.
