Description
Specifications Table
| Product Name | Tryptone Bile Glucuronic Agar | M1591-500 gm Himedia |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Lab Grade |
| Application | Selective medium for E. coli detection |
Product Description
Tryptone Bile Glucuronic Agar (M1591) is a specialized microbiological medium designed for the selective isolation and identification of Escherichia coli (E. coli) in water, food, and clinical samples. This agar contains bile salts and glucuronic acid, which inhibit the growth of gram-positive bacteria while promoting the growth of E. coli. The medium turns yellow due to the fermentation of glucuronic acid, making it easier to identify E. coli colonies.
Whether you’re a student, researcher, or educator, this medium is essential for microbiology labs focusing on bacterial detection and identification. The 500gm pack ensures you have an ample supply for multiple experiments or batch testing.
Looking for reliable lab media? Buy Tryptone Bile Glucuronic Agar online at eqipped for authentic Himedia products.
Key Applications
This agar is widely used in:
- Water quality testing for E. coli contamination
- Food safety analysis to detect fecal coliforms
- Clinical microbiology for diagnostic purposes
- Educational labs for bacterial culture studies
How to Use
To prepare the medium, follow these steps:
- Dissolve 50gm of the powder in 1 liter of distilled water.
- Heat gently while stirring to ensure complete dissolution.
- Autoclave at 121°C for 15 minutes.
- Cool to 45-50°C and pour into sterile Petri dishes.
- Allow the agar to solidify before inoculation.
For best results, store the prepared plates at 2-8°C and use within a week.
Why This Medium Stands Out
Unlike generic agar media, Tryptone Bile Glucuronic Agar offers high selectivity for E. coli due to its unique composition. The presence of glucuronic acid ensures that only E. coli colonies produce a distinct yellow color, making identification straightforward. This reduces false positives and improves accuracy in testing.
Need a trusted supplier for lab media? Shop Tryptone Bile Glucuronic Agar online at eqipped today.
Safety and Handling
While this product is non-hazardous, standard lab safety protocols should be followed:
- Wear gloves and a lab coat when handling.
- Avoid inhalation of powder.
- Dispose of used media as per biohazard waste guidelines.
FAQs
What is the shelf life of Tryptone Bile Glucuronic Agar?
The powder has a shelf life of 24 months when stored in a cool, dry place. Prepared plates should be used within 7 days.
Can this medium detect other bacteria besides E. coli?
It is selective for E. coli, but some other coliforms may grow. Confirmatory tests are recommended for accurate identification.
Is this product suitable for student labs?
Yes, it is widely used in educational settings for microbiology experiments.
How do I confirm E. coli colonies on this agar?
E. coli colonies appear yellow due to glucuronic acid fermentation. Further biochemical tests can be performed for confirmation.
Where can I buy Tryptone Bile Glucuronic Agar online?
You can purchase authentic Himedia M1591 agar from eqipped with fast shipping across India.
