Description
Specifications Table
| Product Name | Tryptone Soya w/ Yeast Extract and Hemin w/o Dextrose |
|---|---|
| Pack Size | 500 gm |
| Form | Powder |
| Grade | Microbiology Grade |
| Application | Bacterial culture, research, and diagnostic microbiology |
Product Description
When it comes to cultivating fastidious microorganisms, the right growth medium makes all the difference. Tryptone Soya w/ Yeast Extract and Hemin w/o Dextrose is a specialized formulation designed to support the growth of demanding bacterial strains, including those requiring additional nutrients like hemin. This medium is widely used in microbiology labs for research, diagnostics, and quality control applications.
Unlike standard tryptone soya agar or broth, this variant includes yeast extract and hemin, which enhance nutrient availability and promote robust bacterial growth. The absence of dextrose makes it suitable for specific metabolic studies where glucose interference is undesirable. Whether you’re working with anaerobic bacteria, fastidious pathogens, or environmental isolates, this medium provides a reliable foundation for your experiments.
At eqipped, we ensure that every batch of this medium meets stringent quality standards, so you can trust its performance in your lab. The 500 gm pack size is ideal for frequent users, offering both convenience and cost-effectiveness.
Key Applications
This medium is particularly useful for:
- Culturing fastidious bacteria like Haemophilus spp. and Neisseria spp.
- Supporting anaerobic bacterial growth in research settings.
- Diagnostic microbiology where enhanced nutrient availability is required.
- Environmental microbiology studies involving nutrient-sensitive strains.
Why This Medium Stands Out
What sets this tryptone soya variant apart is its balanced formulation. The addition of yeast extract provides essential vitamins and amino acids, while hemin supports the growth of bacteria that require iron-containing compounds. The absence of dextrose prevents unwanted fermentation, making it a versatile choice for various microbiological applications.
For researchers and students alike, this medium simplifies the process of culturing challenging microorganisms. Its consistency and reliability make it a staple in labs across India, from academic institutions to industrial research facilities.
How to Use
Preparing this medium is straightforward. Simply dissolve the powder in distilled water, sterilize by autoclaving, and pour into Petri dishes or use as a broth. The recommended concentration is typically 30-40 gm per liter, but always refer to your specific protocol for optimal results.
Store the powder in a cool, dry place, away from direct sunlight. Once prepared, the medium should be used promptly or stored under appropriate conditions to maintain its efficacy.
Safety and Handling
While this medium is generally safe to handle, standard lab practices should be followed. Wear appropriate personal protective equipment (PPE) when preparing and handling the medium. Avoid inhalation of the powder, and ensure proper disposal of used materials as per lab guidelines.
FAQs
What is the difference between tryptone soya agar and this variant?
This variant includes yeast extract and hemin, which provide additional nutrients for fastidious bacteria. It also lacks dextrose, making it suitable for specific metabolic studies.
Can this medium be used for anaerobic bacterial culture?
Yes, the inclusion of hemin supports the growth of anaerobic bacteria, making it ideal for such applications.
What is the shelf life of this product?
The powder has a shelf life of 2-3 years when stored properly. Once prepared, the medium should be used within a few weeks.
Is this medium suitable for clinical diagnostics?
Yes, it is commonly used in diagnostic labs for culturing fastidious pathogens.
Where can I buy tryptone soya medium online in India?
You can buy this high-quality medium online at eqipped, ensuring authentic Himedia products with reliable delivery.
