Specifications Table

Product Name – Water, DEPC treated, Molecular Biology Grade
Quantity/Pack Size – 500 ML
Form – Liquid
Grade – Molecular Biology
Application – RNA/DNA experiments, PCR, qPCR, RT-PCR

Product Overview

DEPC-treated water is an essential reagent for molecular biology applications requiring absolute nuclease-free conditions. This high-purity water undergoes rigorous treatment with diethyl pyrocarbonate (DEPC) to inactivate RNases and DNases, followed by autoclaving to remove residual DEPC. The result is a sterile, endotoxin-free solution that preserves the integrity of nucleic acids during critical procedures like cDNA synthesis, in vitro transcription, and PCR amplification. Its ultra-low conductivity and absence of organic contaminants make it ideal for preparing buffers, diluting enzymes, and reconstituting primers or probes. Unlike standard distilled water, this molecular biology-grade formulation is validated for consistency across batches, ensuring reproducibility in sensitive assays. The 500 mL pack size offers convenience for frequent lab use while maintaining cost efficiency. Store at room temperature (15–25°C) and protect from direct light to maintain stability. Compatible with most downstream applications, this water eliminates the risk of RNA degradation, making it a staple in genomics and proteomics workflows.

FAQs

1. Why is DEPC treatment necessary for this water?

DEPC treatment chemically inactivates RNases and DNases that could degrade nucleic acids during experiments. Autoclaving afterward removes residual DEPC, leaving ultra-pure water safe for molecular biology.

2. Can this water replace nuclease-free water in all applications?

Yes, it meets the same purity standards as commercially labeled “nuclease-free” water and is validated for RNA/DNA work, including PCR, sequencing, and cloning.

3. How should I store opened bottles to prevent contamination?

After opening, keep the bottle tightly sealed at room temperature. Avoid repeated exposure to air by aliquoting into smaller sterile containers if frequent access is needed.

4. Is this water compatible with enzyme-based reactions like restriction digests?

The absence of inhibitors and ions ensures compatibility with most enzymes, including restriction endonucleases, polymerases, and ligases.

5. What’s the shelf life of unopened DEPC-treated water?

Unopened bottles remain stable for 24 months from the date of manufacture when stored under recommended conditions.