Description
Specifications Table
| Product Name | Water, RNase, DNase-free 500ml | S60610.AP | Thermofisher |
|---|---|
| Quantity/Pack Size | 500ML |
| Form | Liquid |
| Grade | Molecular Biology Grade |
| Application | PCR, DNA/RNA extraction, enzyme reactions, cell culture |
Product Description
When precision matters in your lab experiments, using the right water can make all the difference. This RNase, DNase-free water from Thermofisher is specifically designed for molecular biology applications where even trace contaminants can compromise results. Whether you’re setting up PCR reactions, preparing DNA/RNA samples, or conducting sensitive enzyme assays, this high-purity water ensures your experiments remain free from nuclease interference.
Unlike standard distilled or deionized water, this product undergoes rigorous purification processes to eliminate nucleases, endotoxins, and other impurities that could degrade nucleic acids. It’s an essential component for researchers, students, and educators who demand reliability in their protocols. The 500ml pack size is ideal for frequent use in academic and professional labs, offering convenience without sacrificing quality.
At eqipped, we understand the importance of consistent, contamination-free reagents in your workflow. That’s why we source this water directly from Thermofisher, a trusted name in life sciences, so you can focus on your research without worrying about reagent integrity.
Upgrade your lab’s water supply today—order RNase, DNase-free water online and experience the difference in your experimental outcomes.
Why This Water Stands Out
Not all lab-grade water is created equal. Here’s what sets this product apart:
It’s tested and certified to be free of RNase and DNase activity, protecting your precious nucleic acid samples from degradation. The molecular biology grade ensures compatibility with even the most sensitive applications, from qPCR to next-generation sequencing prep.
The low endotoxin levels make it suitable for cell culture applications where purity is non-negotiable. Unlike tap or even some distilled waters, this product won’t introduce variables that could skew your data.
Packaged in sterile, nuclease-free containers, it arrives ready to use—no additional filtration or autoclaving required. This saves time and reduces the risk of contamination during preparation.
Common Applications
This versatile water is a staple in labs working with nucleic acids. You’ll find it indispensable for:
Preparing PCR master mixes where nuclease contamination would amplify unwanted products. Diluting primers, probes, and enzymes without introducing inhibitors. Resuspending DNA/RNA pellets after precipitation. Performing in vitro transcription/translation assays. Setting up restriction enzyme digests and ligation reactions.
Even in educational settings, this water helps students achieve reproducible results in their molecular biology practicals, reinforcing proper lab techniques.
Handling and Storage
To maintain the integrity of this nuclease-free water, follow these best practices:
Store at room temperature (15–25°C) in its original container. Avoid repeated freeze-thaw cycles, as this can compromise the packaging’s sterility. Use sterile technique when aliquoting to prevent contamination. Always use dedicated pipettes or tips that haven’t been exposed to nucleases.
Once opened, use the entire bottle within a reasonable timeframe to minimize exposure to environmental contaminants. For long-term storage, consider aliquoting into smaller, sterile volumes.
Safety Information
While this product is non-hazardous, standard lab safety practices should always be followed:
Wear appropriate PPE (gloves, lab coat) when handling. Avoid ingestion or contact with eyes. In case of spill, wipe with a clean, lint-free cloth and dispose of properly.
FAQs
Is this water autoclaved?
No, this water is purified through ultrafiltration and other specialized processes to remove nucleases. Autoclaving isn’t necessary and could potentially introduce contaminants.
Can I use this water for cell culture?
Yes, the low endotoxin levels make it suitable for most cell culture applications. However, for highly sensitive cell lines, you may want to supplement with additional testing.
How long does this water remain nuclease-free after opening?
When handled aseptically, it remains stable for several weeks. For best results, aliquot into sterile tubes and store at room temperature.
Is this the same as DEPC-treated water?
While both are nuclease-free, this product doesn’t contain DEPC, making it safer for applications where DEPC interference is a concern.
Can I use this water to prepare buffers for molecular biology?
Absolutely. It’s ideal for preparing Tris buffers, TE buffers, and other solutions where nuclease contamination must be avoided.
