Description
Specifications Table
Product Name – Whatman NC45 ST Membrane Filter
Quantity/Pack Size – 100
Diameter – 47MM
Pore Size – 0.45µM
Form – Membrane Disc
Grade – Nitrocellulose
Application – Protein Blotting, Nucleic Acid Transfers
Product Overview
The Whatman NC45 ST nitrocellulose membrane filter is engineered for superior protein binding and retention, making it a trusted choice for critical laboratory applications. With a 0.45µm pore size, this membrane ensures efficient filtration while maintaining high flow rates, reducing processing time without compromising performance. The nitrocellulose composition provides a high binding capacity for proteins, making it particularly effective for Western blotting, dot/slot blotting, and nucleic acid transfers. Its uniform surface and consistent pore distribution guarantee reliable, reproducible results across experiments. The 47MM diameter discs are pre-cut for convenience, eliminating the need for additional handling or trimming. Designed for durability, these filters resist tearing and deformation during handling, ensuring integrity throughout the workflow. The hydrophobic nature of nitrocellulose also minimizes non-specific binding, enhancing signal clarity in downstream applications. Whether used in manual or automated systems, the Whatman NC45 ST delivers consistent performance, reducing variability and improving experimental outcomes. This pack of 100 filters is ideal for laboratories requiring bulk quantities without sacrificing quality or precision.
FAQs
1. What is the protein binding capacity of Whatman NC45 ST filters?
These nitrocellulose membranes typically bind 80-100 µg/cm² of protein, depending on the specific protein and experimental conditions.
2. Can these filters be used with organic solvents?
Yes, Whatman NC45 ST membranes are compatible with many organic solvents, but prolonged exposure may affect structural integrity.
3. How should these filters be stored to maintain performance?
Store in a cool, dry place away from direct sunlight and moisture. Keep the original packaging sealed until use.
4. Are these filters autoclavable?
No, nitrocellulose membranes are not autoclavable as high temperatures can damage the material.
5. What alternatives exist for lower protein binding needs?
For lower binding requirements, consider PVDF membranes, which offer different binding properties and chemical resistance.
