Description
Specifications Table
Product Name – Wright’s stain solution Indicator
Quantity/Pack Size – 125 ml
Form – Liquid (Alcohol-based solution)
Grade – Laboratory/Analytical Grade
Application – Hematology, blood smear staining, microscopic cell differentiation
Product Overview
Wright’s stain solution is a specialized alcohol-based dye formulation designed for high-contrast staining of blood smears, enabling clear visualization of cellular components under a microscope. This laboratory-grade stain combines eosin (a red dye) and methylene blue (a basic dye) in a methanol solvent, creating a balanced pH environment that selectively binds to acidic and basic cell structures. The result is a differential staining pattern where erythrocytes appear pinkish-red, lymphocytes show deep blue nuclei, and granulocytes exhibit distinct granular patterns. The solution is optimized for consistency, minimizing precipitation or clumping that can obscure fine cellular details. Its rapid penetration ensures uniform staining within minutes, reducing processing time without compromising accuracy. The 125 ml pack size offers an ideal balance between volume and shelf stability, with the methanol base acting as a preservative to maintain dye integrity over extended storage periods. Compatible with standard slide preparation protocols, this stain delivers reproducible results across batch variations, making it a reliable choice for routine and research applications where cellular morphology is critical. The formulation adheres to strict quality controls to eliminate particulate contaminants that could interfere with microscopic analysis.
FAQs
1. What is the shelf life of Wright’s stain solution once opened?
The solution remains stable for 12–18 months after opening if stored in a tightly sealed container at room temperature, away from direct sunlight and moisture. Evaporation of the methanol solvent over time may concentrate the dye, so periodic filtration is recommended for optimal performance.
2. Can this stain be used with automated slide stainers?
Yes, the solution is compatible with most automated hematology stainers, but dilution ratios may need adjustment based on the specific instrument’s protocol. Always verify the manufacturer’s guidelines for your equipment, as flow rates and staining cycles can affect the final contrast.
3. How does Wright’s stain differ from Giemsa stain in terms of composition?
Wright’s stain uses a simpler dye mixture of eosin and methylene blue in methanol, while Giemsa stain incorporates additional components like azure B and sometimes glycerol for enhanced nuclear detail. Wright’s tends to produce more vivid cytoplasmic staining, whereas Giemsa offers finer granular differentiation in certain cell types.
4. What precautions should be taken when handling this stain?
The methanol base makes the solution flammable, so avoid open flames and ensure adequate ventilation during use. Wear nitrile gloves and safety goggles to prevent skin or eye contact, as the dyes can cause irritation. Dispose of waste stain according to local biohazard or chemical waste regulations.
5. Why does my stained smear show uneven color distribution?
Uneven staining typically results from improper slide fixation, insufficient staining time, or pH imbalance in the buffer. Ensure slides are air-dried completely before staining, and use a fresh, filtered stain solution. Adjusting the pH of the rinsing buffer (neutral pH 6.8–7.2) can also improve uniformity.
