Description
Specifications Table
| Product Name | Xylene Cyanol FF C.I. 43535 | 327705 | CDH |
|---|---|
| Pack Size | 25g |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Electrophoresis, DNA/RNA staining |
Product Description
Xylene Cyanol FF is a synthetic dye widely used in molecular biology laboratories as a tracking dye during gel electrophoresis. Its deep blue color makes it easy to visualize DNA or RNA migration in agarose or polyacrylamide gels. This high-quality dye from CDH ensures consistent performance, helping researchers achieve accurate results in their experiments.
Whether you’re a student setting up your first gel or a seasoned researcher optimizing protocols, Xylene Cyanol FF provides reliable tracking without interfering with downstream applications. Its water-soluble nature allows for easy preparation of loading buffers, and its stability ensures long shelf life when stored properly.
At eqipped, we understand the importance of precision in lab work. That’s why we source Xylene Cyanol FF from trusted manufacturers like CDH, ensuring you receive a product that meets rigorous quality standards. Our streamlined ordering process makes it simple to buy Xylene Cyanol FF online, with fast delivery to your lab or institution anywhere in India.
Key Applications
Xylene Cyanol FF serves multiple purposes in molecular biology:
- Tracking dye in agarose gel electrophoresis for DNA fragments
- Visual marker in polyacrylamide gels for RNA analysis
- Component in DNA loading buffers for sample preparation
- Quality control in nucleic acid purification protocols
Safety and Handling
While Xylene Cyanol FF is generally safe when handled properly, it’s important to follow standard laboratory safety practices. Always wear appropriate personal protective equipment (PPE) including gloves and safety goggles when handling the powder. Store the dye in a cool, dry place away from direct sunlight to maintain its stability.
Why Researchers Trust Xylene Cyanol FF
This particular tracking dye has become a staple in labs worldwide due to its consistent performance. Unlike some alternatives, Xylene Cyanol FF maintains its color intensity throughout electrophoresis runs, making it easier to monitor progress. Its migration rate in standard agarose gels (approximately 5 kb DNA) provides a reliable reference point for estimating fragment sizes.
For educational institutions, Xylene Cyanol FF offers an excellent balance between affordability and quality. Students can clearly observe gel loading and migration patterns, making it an invaluable teaching tool in biology and biochemistry courses.
Choosing the Right Supplier
When looking to buy Xylene Cyanol FF online, it’s crucial to select a supplier that prioritizes quality and authenticity. eqipped partners with reputable manufacturers to ensure you receive genuine CDH products. Our transparent sourcing and competitive pricing make us a preferred choice among Indian researchers and educators.
Storage and Stability
To maximize shelf life, store Xylene Cyanol FF in its original container at room temperature. Keep the container tightly sealed when not in use to prevent moisture absorption. Properly stored, this dye maintains its effectiveness for extended periods, making it a cost-effective choice for laboratories.
Preparation Tips
For optimal results, prepare a 0.05% solution by dissolving 50 mg of Xylene Cyanol FF in 100 ml of distilled water or appropriate buffer. This concentration provides sufficient visibility without overloading your gel. Always filter the solution before use to remove any undissolved particles that might interfere with your electrophoresis.
FAQs
What is the migration rate of Xylene Cyanol FF in agarose gels?
Xylene Cyanol FF typically migrates at approximately 5 kb in standard 1% agarose gels, making it useful for tracking larger DNA fragments.
Can Xylene Cyanol FF be used for RNA electrophoresis?
Yes, it’s commonly used in both DNA and RNA gel electrophoresis as a visual tracking dye.
How should I dispose of Xylene Cyanol FF waste?
Follow your institution’s chemical waste disposal guidelines. Small quantities can often be disposed of as general laboratory waste.
Is Xylene Cyanol FF compatible with all gel staining methods?
Yes, it doesn’t interfere with common DNA stains like ethidium bromide or SYBR Safe.
What’s the shelf life of Xylene Cyanol FF when stored properly?
When stored in a cool, dry place in its original container, it typically remains stable for 2-3 years.
