Description
Specifications Table
Product Name – Whatman NC20 Membrane Filter
Quantity/Pack Size – 47MM, 0.2uM (Pack of 100) | 50MM, 0.2uM (Pack of 100)
Form – Circular membrane discs
Grade – Analytical/Research
Application – Sterile filtration, particle retention, microbiological analysis
Product Overview
The Whatman NC20 Membrane Filter is a high-performance nitrocellulose membrane designed for critical laboratory filtration applications. With a precise 0.2?m pore size, it ensures reliable retention of microorganisms, particles, and contaminants, making it ideal for sterile filtration processes. The nitrocellulose composition provides excellent protein binding capacity (up to 80-100 ?g/cm?), which is particularly advantageous for applications requiring protein adsorption. This membrane is chemically compatible with a wide range of solvents, acids, and bases, ensuring versatility across different laboratory protocols. Its uniform pore structure guarantees consistent flow rates and high loading capacity, reducing the risk of clogging during filtration. The NC20 membrane is also hydrophilic, allowing for rapid wetting and efficient filtration without the need for pre-treatment. Available in standard 47MM and 50MM diameters, these filters are packaged in sterile conditions to maintain integrity until use. The white, opaque appearance of the membrane allows for easy visualization of retained particles, aiding in qualitative analysis. Whether used in research, quality control, or routine lab procedures, the Whatman NC20 delivers reproducibility and accuracy, backed by Whatman?s reputation for filtration excellence.
FAQs
1. What is the protein binding capacity of the Whatman NC20 membrane filter?
The Whatman NC20 membrane has a protein binding capacity of approximately 80-100 ?g/cm?, making it suitable for applications requiring protein adsorption or immobilization.
2. Can this filter be used with organic solvents like acetone or methanol?
Yes, the nitrocellulose material is compatible with many organic solvents, including acetone, methanol, ethanol, and DMSO, but always verify compatibility for your specific solvent concentration.
3. How does the 0.2?m pore size compare to other common membrane filters?
A 0.2?m pore size is standard for sterile filtration, effectively removing bacteria, yeast, and most particles while allowing liquids and smaller molecules to pass through. It?s finer than 0.45?m filters but coarser than 0.1?m membranes.
4. Is autoclaving required before use for sterile applications?
The Whatman NC20 membranes are typically supplied sterile if labeled as such. However, if sterility is critical for your application, autoclaving at 121?C for 15-20 minutes is recommended to ensure complete sterilization.
5. What alternatives exist if I need a filter with lower protein binding?
For applications requiring minimal protein binding, consider PES (polyethersulfone) or PVDF (polyvinylidene fluoride) membranes, which offer lower protein adsorption while maintaining high chemical resistance.
